Home | Publications
[2019]
Formate oxidase (FOx) from Aspergillus oryzae: one catalyst to promote H2O2‐dependent biocatalytic oxidation reactions
Tieves F, Willot SJ-P, van Schie MMCH, Rauch MCR, Younes SHH, Zhang W, Dong J, Gómez de Santos P, Robbins JM, Bommarius B, Alcalde M, Bommarius AS, Hollmann F

Angew. Chem. Int. Ed., 58: 7873-7877, 10.1002/anie.201902380


An increasing number of biocatalytic oxidation reactions rely on H2O2 as clean oxidant. The poor robustness of most enzymes towards H2O2, however, necessitates more efficient in situ H2O2 generation systems. In analogy to the well‐known formate dehydrogenase to promote NADH‐dependent reactions, we here propose employing formate oxidase (FOx) to promote H2O2‐dependent enzymatic oxidation reactions. Already under non‐optimised conditions, high turnover numbers for coupled peroxygenase‐FOx catalysis have been achieved. PDF

External link